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Addgene inc pcas9 cat
Pcas9 Cat, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/u6+sguprt/pSAG1%3A%3ACAS9-U6%3A%3AsgUPRT+(Plasmid+%2354467)/bio_rxiv__64898__2026__01__22__701003-272-14-15
Average 93 stars, based on 61 article reviews
pcas9 cat - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Recombinant:

Article Title: Toxoplasma bradyzoites exhibit physiological plasticity of calcium and energy stores controlling motility and egress
Article Snippet: Recombinant DNA reagent , pBAG1:mCherry, SAG1:CAT, TUB1:GCaM6f (pNJ-26) , This paper , , Generation of BAG1-mCherry GCaMP6f reporter. .. Recombinant DNA reagent , pSAG1:CAS9-EGFP, U6:sgUPRT , Addgene , Addgene_54467 , Template for construction of pSAG1:CAS9-GFP, U6:sgDHFR 3’UTR. .. Recombinant DNA reagent , pSAG1:CAS9-EGFP, U6:sgDHFR 3’UTR , This paper , , Generation of ratiometric reporter.

CRISPR:

Article Title: Phospho-relay feedback loops control egress vs. intracellular development in Toxoplasma gondii.
Article Snippet: .. CRISPR/CAS9 plasmids designed to target specific genes were generated frompSAG1:CAS9-GFP, U6:sgUPRT (Shen et al., 2014; Addgene #54467) by replacing the single guide sgUPRTwith sgPKAc1, sgPDE2, or sgHXGPRT using HiFi assembly. ..

Article Title: Phospho-relay feedback loops control egress vs. intracellular development in Toxoplasma gondii
Article Snippet: .. CRISPR/CAS9 plasmids designed to target specific genes were generated from p SAG1:CAS9-GFP, U6:sgUPRT (Shen et al., 2014; Addgene #54467) by replacing the single guide sgUPRT with sgPKAc1, sgPDE2 , or sgHXGPRT using HiFi assembly. ..

Generated:

Article Title: Phospho-relay feedback loops control egress vs. intracellular development in Toxoplasma gondii.
Article Snippet: .. CRISPR/CAS9 plasmids designed to target specific genes were generated frompSAG1:CAS9-GFP, U6:sgUPRT (Shen et al., 2014; Addgene #54467) by replacing the single guide sgUPRTwith sgPKAc1, sgPDE2, or sgHXGPRT using HiFi assembly. ..

Article Title: A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability
Article Snippet: This plasmid (pACT1:Cas9-GFP) was further modified by inserting the thymidine kinase (TK, cgd5_4440) guide RNA (sgRNA) and the tracrRNA amplified from the Aldolase_Cas9 plasmid ( ) downstream of the CpU6 promoter using Gibson assembly to create pACT1:Cas9-GFP, U6:sgTK (Addgene 122852). .. The plasmid pACT1:Cas9-GFP, U6:sgUPRT (Addgene 122853) was generated by replacing the TK sgRNA with a sgRNA targeting the C. parvum uracil phosphoribosyltransferase gene ( uprt , cgd1_1900) using Q5 site-directed mutagenesis (New England Biosciences). ..

Article Title: Phospho-relay feedback loops control egress vs. intracellular development in Toxoplasma gondii
Article Snippet: .. CRISPR/CAS9 plasmids designed to target specific genes were generated from p SAG1:CAS9-GFP, U6:sgUPRT (Shen et al., 2014; Addgene #54467) by replacing the single guide sgUPRT with sgPKAc1, sgPDE2 , or sgHXGPRT using HiFi assembly. ..

Plasmid Preparation:

Article Title: A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability
Article Snippet: This plasmid (pACT1:Cas9-GFP) was further modified by inserting the thymidine kinase (TK, cgd5_4440) guide RNA (sgRNA) and the tracrRNA amplified from the Aldolase_Cas9 plasmid ( ) downstream of the CpU6 promoter using Gibson assembly to create pACT1:Cas9-GFP, U6:sgTK (Addgene 122852). .. The plasmid pACT1:Cas9-GFP, U6:sgUPRT (Addgene 122853) was generated by replacing the TK sgRNA with a sgRNA targeting the C. parvum uracil phosphoribosyltransferase gene ( uprt , cgd1_1900) using Q5 site-directed mutagenesis (New England Biosciences). ..

Article Title: A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability
Article Snippet: Plasmid: pACT1:Cas9-GFP, U6:sgTK , This paper; Addgene , Cat# 122852. .. Plasmid: pACT1:Cas9-GFP, U6:sgUPRT , This paper; Addgene , Cat# 122853. .. Plasmid: pUC19 vector , New England Biolabs, Inc. , Cat# N3041S.

Mutagenesis:

Article Title: A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability
Article Snippet: This plasmid (pACT1:Cas9-GFP) was further modified by inserting the thymidine kinase (TK, cgd5_4440) guide RNA (sgRNA) and the tracrRNA amplified from the Aldolase_Cas9 plasmid ( ) downstream of the CpU6 promoter using Gibson assembly to create pACT1:Cas9-GFP, U6:sgTK (Addgene 122852). .. The plasmid pACT1:Cas9-GFP, U6:sgUPRT (Addgene 122853) was generated by replacing the TK sgRNA with a sgRNA targeting the C. parvum uracil phosphoribosyltransferase gene ( uprt , cgd1_1900) using Q5 site-directed mutagenesis (New England Biosciences). ..



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